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KMID : 1007520130220061741
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Food Science and Biotechnology
2013 Volume.22 No. 6 p.1741 ~ p.1745
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Angiotensin Converting Enzyme Inhibitory Benzopyranoids from Angelica gigas
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Hyun Sook-Kyung
Oh You-Na
Kwon Hyun-Ju
Kim Byung-Woo
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Abstract
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Angiotensin converting enzyme (ACE) plays a major role in the regulation of blood pressure. The potential antihypertensive activity of Angelica gigas was evaluated in vitro by its ability to inhibit ACE. A methanol extract of A. gigas roots exerted an inhibitory effect against ACE and was fractionated using several organic solvents, including dichloromethane, ethyl acetate, and n-butanol. The n-butanol soluble fraction, which manifested potent ACE inhibitory properties, was further purified via repeated silica gel and RP-18 column chromatography. Three benzopyriansides, such as nodakenin (1), umbelliferone (2), and umbelliferone 6-carboxylic acid (3) were isolated from A. gigas roots. Compounds 1-3 inhibited ACE activity in a dose-dependent manner, with IC50 values of 63.95±0.16, 190.49±0.54, and 158.35±1.53 μM, respectively. The inhibition kinetics analyzed by Lineweaver-Burk plots revealed that the three compounds showed a mixed-type inhibition. The equilibrium constants for the inhibitor binding (Ki) of 1, 2, and 3 were estimated to be 1.56×10?4, 4.70×10?4, and 2.17×10?4 M, respectively. We used A. gigas roots to isolate nodakinin, umbelliferone, and umbelliferone 6-carboxylic acid and tested their inhibition efficacy against ACE for the first time.
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KEYWORD
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angiotensin converting enzyme, nodakenin, umbelliferone, umbelliferone 6-carboxylic acid, Agelica gigas
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